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rosettesep™ human cd4- cd8-depletion cocktails  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc rosettesep™ human cd4- cd8-depletion cocktails
    a , The effect of Adam2 overexpression in LLC cells on <t>CD8</t> + OT-I T cell mediated killing. CTRL or Adam2 O/E cells labeled with CFSE were used as targets (T) and cocultured with day 4 activated and expanded OT-I CD8 T-cells (E) at different E:T ratios. Flow cytometry analysis was used to calculate the ratio of killed cells at different E:T ratios. Data presents mean ± s.e.m. of three replicates and analysed by two-sided student’s t-test. b , Tumor growth of transplanted CTLR or Adam2 O/E in B6 mice, untreated (n=5) or treated with OT-I T cells (n=5). c , Volcano plot showing differentially expressed genes between OT-I treated CTLR and Adam2 O/E tumors isolated from B6 mice. d , GSEA plots showing Adipogenesis and Peroxisomes pathways in CTLR tumors versus Adam2 O/E tumors. e and f , Bar graph showing changes of Gene Ontology of Biological Process (e) and Molecular Function (f) between OT-I treated CTLR versus Adam2 O/E tumors.
    Rosettesep™ Human Cd4 Cd8 Depletion Cocktails, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rosettesep+human+cd4+and+cd8+depletion+cocktail/bio_rxiv__2022__03__13__484176-335-16-18?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    rosettesep™ human cd4- cd8-depletion cocktails - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer"

    Article Title: In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer

    Journal: bioRxiv

    doi: 10.1101/2022.03.13.484176

    a , The effect of Adam2 overexpression in LLC cells on CD8 + OT-I T cell mediated killing. CTRL or Adam2 O/E cells labeled with CFSE were used as targets (T) and cocultured with day 4 activated and expanded OT-I CD8 T-cells (E) at different E:T ratios. Flow cytometry analysis was used to calculate the ratio of killed cells at different E:T ratios. Data presents mean ± s.e.m. of three replicates and analysed by two-sided student’s t-test. b , Tumor growth of transplanted CTLR or Adam2 O/E in B6 mice, untreated (n=5) or treated with OT-I T cells (n=5). c , Volcano plot showing differentially expressed genes between OT-I treated CTLR and Adam2 O/E tumors isolated from B6 mice. d , GSEA plots showing Adipogenesis and Peroxisomes pathways in CTLR tumors versus Adam2 O/E tumors. e and f , Bar graph showing changes of Gene Ontology of Biological Process (e) and Molecular Function (f) between OT-I treated CTLR versus Adam2 O/E tumors.
    Figure Legend Snippet: a , The effect of Adam2 overexpression in LLC cells on CD8 + OT-I T cell mediated killing. CTRL or Adam2 O/E cells labeled with CFSE were used as targets (T) and cocultured with day 4 activated and expanded OT-I CD8 T-cells (E) at different E:T ratios. Flow cytometry analysis was used to calculate the ratio of killed cells at different E:T ratios. Data presents mean ± s.e.m. of three replicates and analysed by two-sided student’s t-test. b , Tumor growth of transplanted CTLR or Adam2 O/E in B6 mice, untreated (n=5) or treated with OT-I T cells (n=5). c , Volcano plot showing differentially expressed genes between OT-I treated CTLR and Adam2 O/E tumors isolated from B6 mice. d , GSEA plots showing Adipogenesis and Peroxisomes pathways in CTLR tumors versus Adam2 O/E tumors. e and f , Bar graph showing changes of Gene Ontology of Biological Process (e) and Molecular Function (f) between OT-I treated CTLR versus Adam2 O/E tumors.

    Techniques Used: Over Expression, Labeling, Flow Cytometry, Isolation



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    a , The effect of Adam2 overexpression in LLC cells on <t>CD8</t> + OT-I T cell mediated killing. CTRL or Adam2 O/E cells labeled with CFSE were used as targets (T) and cocultured with day 4 activated and expanded OT-I CD8 T-cells (E) at different E:T ratios. Flow cytometry analysis was used to calculate the ratio of killed cells at different E:T ratios. Data presents mean ± s.e.m. of three replicates and analysed by two-sided student’s t-test. b , Tumor growth of transplanted CTLR or Adam2 O/E in B6 mice, untreated (n=5) or treated with OT-I T cells (n=5). c , Volcano plot showing differentially expressed genes between OT-I treated CTLR and Adam2 O/E tumors isolated from B6 mice. d , GSEA plots showing Adipogenesis and Peroxisomes pathways in CTLR tumors versus Adam2 O/E tumors. e and f , Bar graph showing changes of Gene Ontology of Biological Process (e) and Molecular Function (f) between OT-I treated CTLR versus Adam2 O/E tumors.
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    Image Search Results


    a , The effect of Adam2 overexpression in LLC cells on CD8 + OT-I T cell mediated killing. CTRL or Adam2 O/E cells labeled with CFSE were used as targets (T) and cocultured with day 4 activated and expanded OT-I CD8 T-cells (E) at different E:T ratios. Flow cytometry analysis was used to calculate the ratio of killed cells at different E:T ratios. Data presents mean ± s.e.m. of three replicates and analysed by two-sided student’s t-test. b , Tumor growth of transplanted CTLR or Adam2 O/E in B6 mice, untreated (n=5) or treated with OT-I T cells (n=5). c , Volcano plot showing differentially expressed genes between OT-I treated CTLR and Adam2 O/E tumors isolated from B6 mice. d , GSEA plots showing Adipogenesis and Peroxisomes pathways in CTLR tumors versus Adam2 O/E tumors. e and f , Bar graph showing changes of Gene Ontology of Biological Process (e) and Molecular Function (f) between OT-I treated CTLR versus Adam2 O/E tumors.

    Journal: bioRxiv

    Article Title: In vivo CRISPR screens reveal Serpinb9 and Adam2 as regulators of immune therapy response in lung cancer

    doi: 10.1101/2022.03.13.484176

    Figure Lengend Snippet: a , The effect of Adam2 overexpression in LLC cells on CD8 + OT-I T cell mediated killing. CTRL or Adam2 O/E cells labeled with CFSE were used as targets (T) and cocultured with day 4 activated and expanded OT-I CD8 T-cells (E) at different E:T ratios. Flow cytometry analysis was used to calculate the ratio of killed cells at different E:T ratios. Data presents mean ± s.e.m. of three replicates and analysed by two-sided student’s t-test. b , Tumor growth of transplanted CTLR or Adam2 O/E in B6 mice, untreated (n=5) or treated with OT-I T cells (n=5). c , Volcano plot showing differentially expressed genes between OT-I treated CTLR and Adam2 O/E tumors isolated from B6 mice. d , GSEA plots showing Adipogenesis and Peroxisomes pathways in CTLR tumors versus Adam2 O/E tumors. e and f , Bar graph showing changes of Gene Ontology of Biological Process (e) and Molecular Function (f) between OT-I treated CTLR versus Adam2 O/E tumors.

    Article Snippet: DNTs were enriched by depleting CD4 + and CD8 + cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies).

    Techniques: Over Expression, Labeling, Flow Cytometry, Isolation